Journal: Non-coding RNA Research
Article Title: Maternal plasma microRNAs as potential biomarkers for triaging pregnancies of unknown location and ectopic pregnancy diagnosis
doi: 10.1016/j.ncrna.2025.05.005
Figure Lengend Snippet: Eight discriminatory miRNAs in early pregnancy plasma when comparing VIUP with EP (N = 15) in discovery study. nCounter probe counts for A: hsa-miR-222-3p, B: hsa-miR-20a-5p + hsa-miR-20b-5p, C: hsa-miR-21-5p, D: hsa-miR-185-5p, E: hsa-miR-148a-3p, F: hsa-miR-19b-3p, G: hsa-miR-1285-5p, and H: hsa-miR-22-3p detected in human plasma in women in early pregnancy who subsequently had a confirmed VIUP (N = 8, green), EP (N = 7, red), or NVIUP (N = 7, purple) ∗Statistical significance of differences between groups was tested using Nanostring nSolver platform. Graphs show geometric mean with geometric standard deviation and p -values, with outcome on x axis, and log(10) counts on y axis. Only statistically significant differences are presented. Abbreviations: VIUP = Viable intrauterine pregnancy; EP = Ectopic pregnancy; NVIUP = Non-viable intrauterine pregnancy.
Article Snippet: Reverse transcription of the extracted RNA samples was achieved using the miRCURY LNA Universal RT miRNA cDNA synthesis kits following the manufacturer's instructions (Qiagen).
Techniques: Clinical Proteomics, Standard Deviation